OBJECTIVE LIFE SCIENCE
BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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5’GGATCC3’
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5’GAATTC3’
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5’AAGCTT3’
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5’CCCGGG3’
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Detailed explanation-1: -The restriction endonuclease EcoRI recognises and cleaves DNA sequence as shown below : 5’-GAATTC-3’ 3’-CTTAAG-5’
Detailed explanation-2: -EcoRI recognizes the sequence GAATTC, and cuts both DNA strands between the G and the A nucleotides.
Detailed explanation-3: -It is a type II restriction enzyme that cuts only at the restriction site with the 5’-GAATTC-3’ sequence. It chops the DNA at a specified location, resulting in sticky ends. EcoRI has a palindrome recognition site that cuts DNA following G, creating sticky ends with AATT.
Detailed explanation-4: -In molecular biology, it is a commonly used restriction enzyme. It creates blunt ends. The enzyme recognizes the palindromic 6-base DNA sequence 5’-GAT|ATC-3’ and makes a blunt end at the vertical line.
Detailed explanation-5: -The restriction endonuclease enzyme EcoRI recognizes the ssDNA sequence 5’-GAATTC’-3, and introduces a single-strand cut between the G & A nucleotides.