OBJECTIVE LIFE SCIENCE
BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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DNA sequencing
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identification of endangered species
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recombinant insulin
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None of the above
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Detailed explanation-1: -An American company named Eli Lilly in 1983, prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin.
Detailed explanation-2: -Recombinant insulin is used as supplement in the culture media for many cell lines, such as CHO, HEK 293 or Sf9, for the manufacturing of monoclonal antibodies, virus vaccines, or gene therapy products, for example.
Detailed explanation-3: -Overview. Recombinant human insulin was one of the first products of biotechnology. It was developed in response to the need for a consistent and sufficient worldwide supply. Recombinant human insulin replaced the animal insulins and semisynthetic insulins obtained by modification of animal insulins.
Detailed explanation-4: -Recombinant Insulin. Gene Therapy. Molecular Diagnosis. Pharmacogenomics. Vaccines.