OBJECTIVE LIFE SCIENCE
BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Which steps are in the correct order to create insulin-producing bacteria?
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cut bacterial plasmid with restriction enzyme, insert human insulin-producing gene, seal with ligase enzyme
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seal with ligase enzyme, cut bacterial plasmid with restriction enzyme, insert human insulin-producing gene
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cut bacterial plasmid with restriction enzyme, seal with ligase enzyme, insert human insulin-producing gene
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insert human insulin-producing gene, cut bacterial plasmid with restriction enzyme, seal with ligase enzyme
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Explanation:
Detailed explanation-1: -insert the human insulin gene into the plasmid. Researchers return the plasmid to the bacteria and… put the “recombinant” bacteria in large fermentation tanks. There, the recombinant bacteria use the gene to begin producing human insulin.
Detailed explanation-2: -Human insulin is extracted from pancreas cells and an insulin-producing gene is isolated. A plasmid DNA is extracted from a bacterium and cut with restriction enzyme, forming plasmid vector. More items
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