LIFE SCIENCE

Detailed explanation-1: -Restriction enzymes cut through both nucleotide strands, breaking the DNA into fragments, but they don’t always do this in the same way. SmaI is an example of a restriction enzyme that cuts straight through the DNA strands, creating DNA fragments with a flat or blunt end .

Detailed explanation-2: -The restriction enzymes generate two different types of cuts. Blunt ends are produced when they cut the DNA at the centre of the recognition sequence, and sticky ends produce an overhang.