LIFE SCIENCE

Detailed explanation-1: -(iii) Hind II was the first restriction endonuclease to be isolated. (iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA. (v) Presence of more than one recognition site for the same restriction enzyme within the vector complicates gene cloning.

Detailed explanation-2: -The first restriction nuclease characterized was isolated from Haemophilus influenzae bacteria. The enzyme (HindII) cuts at a particular site within a specific sequence of six base pairs as follows. (Where nucleotides are shown in parenthesis, the enzyme recognizes either one of the two bases shown.)

Detailed explanation-3: -HindII was the first restriction enzyme to be isolated. This enzyme was first isolated from Haemophilus influenzae Rd strain II. So, the enzyme was shorts labeled as HindII. Each enzyme is named by a three-letter code in italics.

Detailed explanation-4: -(Science: enzyme molecular biology) first type II restriction endonuclease identified, by Hamilton Smith in 1970. Isolated from haemophilus influenzae, it cleaves the sequence GTPyPuAC between the unspecified pyrimidine and purine generating blunt ends.

Detailed explanation-5: -Hamilton O. Smith was the first person to isolate the restrictive enzyme from the bacterium Haemophilus influenzae. The first enzyme to be isolated was HindII and it could cut from the specific six-base pair.