LIFE SCIENCE

Detailed explanation-1: -Metaphase. Chromosomes line up at the metaphase plate, under tension from the mitotic spindle. The two sister chromatids of each chromosome are captured by microtubules from opposite spindle poles. In metaphase, the spindle has captured all the chromosomes and lined them up at the middle of the cell, ready to divide.

Detailed explanation-2: -Metaphase II: During metaphase, each of the 23 chromosomes line up along the center of the cell at the metaphase plate.

Detailed explanation-3: -In metaphase II, the chromosomes line up individually along the metaphase plate. In anaphase II, the sister chromatids separate and are pulled towards opposite poles of the cell.

Detailed explanation-4: -During mitosis, chromosomes are bound to microtubules emanating from both poles of the mitotic spindle via sister-kinetochores and aligned on the metaphase plate precisely in the middle of the spindle.