OBJECTIVE LIFE SCIENCE
CELL BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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low power objective
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medium power objective
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high power objective
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stage
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Detailed explanation-1: -The higher the power of magnification, the smaller the field of view. For example, if you determine that your field of view is 2.5 mm in diameter using a 10X ocular and 4X objective, you will be able to determine what the field of view will be with the high power objective by using the above formula.
Detailed explanation-2: -A high-power field (HPF), when used in relation to microscopy, references the field of view under the maximum magnification power of the objective being used. Often, this represents a 400-fold magnification when referenced in scientific papers.
Detailed explanation-3: -The field of view is largest on the lowest power objective. When you switch to a higher power, the field of view closes in towards the center. You will see more of an object on low power. Therefore, it is best to find an object on low power, center it, and then switch to the next higher power and repeat.