OBJECTIVE LIFE SCIENCE
EVOLUTION
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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to clone chromosomes
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to cut DNA into fragments
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to inject recombinant DNA into cells
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to separate DNA fragments by size
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Detailed explanation-1: -Electrophoresis enables you to distinguish DNA fragments of different lengths. DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively charged electrode.
Detailed explanation-2: -Because DNA has a uniform mass/charge ratio, DNA molecules are separated by size within an agarose gel in a pattern such that the distance traveled is inversely proportional to the log of its molecular weight(3).
Detailed explanation-3: -These DNA size standards can be used as positive controls for electrophoresis and for determining the sizes of unknown DNA fragments.
Detailed explanation-4: -Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size.
Detailed explanation-5: -DNA gel electrophoresis is a technique used for the detection and separation of DNA molecules. An electric field is applied to a gel matrix comprised of agarose, and within the gel, charge particles will migrate and separate based on size.