OBJECTIVE LIFE SCIENCE
EVOLUTION
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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locate the desired piece of DNA in the donor organism
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insert the desired piece of DNA into the bacterial plasmid
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get the bacteria to reproduce with the new, desired gene in them
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use the plasmid as a vector to get the desired piece of DNA into the host organism
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Detailed explanation-1: -In a typical cloning experiment, researchers first insert a piece of DNA, such as a gene, into a circular piece of DNA called a plasmid. This step uses restriction enzymes and DNA ligase and is called a ligation. After a ligation, the next step is to transfer the DNA into bacteria in a process called transformation.
Detailed explanation-2: -Step 1: DNA Extraction DNA extraction is the first step in the genetic engineering process. In order to work with DNA, scientists must extract it from the desired organism. A sample of an organism containing the gene of interest is taken through a series of steps to remove the DNA.
Detailed explanation-3: -A transgenic, or genetically modified, organism is one that has been altered through recombinant DNA technology, which involves either the combining of DNA from different genomes or the insertion of foreign DNA into a genome.
Detailed explanation-4: -Locating an organism with a specific trait and extracting its DNA. Cloning a gene that controls the trait. Designing a gene to express in a specific way. Transformation, inserting the gene into the cells of a crop plant. Cross the transgene into an elite background.