OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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A restriction enzyme is used to cut both the gene of interest and the plasmid at the restriction sites.
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True
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False
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Either A or B
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None of the above
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Explanation:
Detailed explanation-1: -The restriction enzymes cuts at fixed sequences, therefore same restriction enzyme must be used since it forms fragments with complementary sticky ends, therefore facilitating the formation of bonds between them.
Detailed explanation-2: -When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes.
Detailed explanation-3: -In the laboratory, restriction enzymes (or restriction endonucleases) are used to cut DNA into smaller fragments. The cuts are always made at specific nucleotide sequences. Different restriction enzymes recognise and cut different DNA sequences.
There is 1 question to complete.