LIFE SCIENCE

OBJECTIVE LIFE SCIENCE

MOLECULAR BIOLOGY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
A student isolates, purifies, and combines in a test tube a variety of molecules needed for DNA replication. After adding some DNA to the mixture, replication occurs, but each DNA molecule consists of a normal strand paired with numerous segments of DNA a few hundred nucleotides long. What has the student probably left out of the mixture?
A
DNA polymerase
B
DNA ligase
C
Okazaki fragments
D
primase
Explanation: 

Detailed explanation-1: -When she adds some DNA to the mixture, replication occurs, but each DNA molecule consists of a normal strand paired with numerous segments of DNA a few hundred nucleotides long. What has she probably left out of the mixture? DNA polymerase.

Detailed explanation-2: -Justification of correct answer. Option (c) is a DNA polymerase. The reverse ( 3’ to 5’ ) exonuclease activity of the DNA polymerase enzyme regulates the proofreading process, allowing them to delete improperly inserted bases from newly produced DNA and replace them with correct bases.

Detailed explanation-3: -Answer and Explanation: The enzyme that is involved in separating the DNA strands during DNA replication is called DNA helicase.

There is 1 question to complete.