LIFE SCIENCE

Detailed explanation-1: -In molecular biology, complementary DNA (cDNA) is synthesised from an RNA template in a reaction catalysed by the enzyme reverse transcriptase (RTase). cDNA synthesis is the first step in many molecular biology workflows, such as gene expression studies using real-time PCR.

Detailed explanation-2: -cDNA is produced in two basic steps: (a) first, mRNA is isolated from other cellular RNA using an elution column. (b) Second, the enzyme reverse transcriptase synthesizes strands of DNA using the mRNA molecules as templates.

Detailed explanation-3: -Complementary DNA (cDNA) is synthesized in a reverse transcription reaction by the enzyme reverse transcriptase from a single-stranded mRNA template.

Detailed explanation-4: -Prepare sample. RNA serves as the template in cDNA synthesis. Remove genomic DNA. Trace amounts of genomic DNA (gDNA) may be co-purified with RNA. Select reverse transcriptase. Prepare reaction mix. Perform cDNA synthesis. Prepare sample. Remove genomic DNA. Select reverse transcriptase. More items