OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Gel electrophoresis
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The method of copying strands of DNA
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The method for cutting strands of DNA
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The method for separating strands of DNA
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None of the above
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Explanation:
Detailed explanation-1: -To separate DNA using agarose gel electrophoresis, the DNA is loaded into pre-cast wells in the gel and a current applied. The phosphate backbone of the DNA (and RNA) molecule is negatively charged, therefore when placed in an electric field, DNA fragments will migrate to the positively charged anode.
Detailed explanation-2: -Preparing the samples for running. An agarose TAE gel solution is prepared. Casting the gel. Setting up the electrophoresis chamber. Loading the gel. Electrophoresis. Stopping electrophoresis and visualizing the DNA. 18-Jun-2019
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