OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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to join pieces of DNA from different sources
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to destroy DNA
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To cut the DNA using restriction enzymes
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None of the above
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Detailed explanation-1: -The sticky ends of the two fragments stick together by complementary base pairing. However, there are still gaps in the sugar-phosphate backbones of the DNA double helix at the junction sites where the gene and plasmid DNA meet. Once they are joined by ligase, the fragments become a single piece of unbroken DNA.
Detailed explanation-2: -Sticky ends are pieces of DNA fragments with short strands on each side that are complementary to each other (because the sequences are palindromes). Sticky ends that are very long can also be called cohesive ends. Sticky ends have overhang basepairs because of the way the restriction enzyme cuts the DNA.
Detailed explanation-3: -Sticky ends can be joined by using DNA ligases. Each restriction enzyme functions by inspecting the length of a DNA sequence.