OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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If a solvent moves 8 cm up a piece of chromatography paper and a pigment moves 5 cm, what is the piment’s retention factor (Rf)?
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0.63
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0.40
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0.16
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1.60
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Explanation:
Detailed explanation-1: -The Retention factor or Rf value applies to chromatography to make the technique scientific. It is defined as the distance travelled by the compound divided by the distance travelled by the solvent. Rf value = Distance travelled by the compound / Distance travelled by the solvent.
Detailed explanation-2: -The Rf value of a compound is equal to the distance traveled by the compound divided by the distance traveled by the solvent front (both measured from the origin).
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