OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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In gel electrophoresis the ____ charge is close to the wells and the ____ charge is on the opposite end because phosphates are ____
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positive, negative, positive
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positive, positive, negative
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negative, positive, negative
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positive, negative, negative
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Explanation:
Detailed explanation-1: -The end of the gel with the wells is positioned towards the negative electrode. The end without wells (towards which the DNA fragments will migrate) is positioned towards the positive electrode.
Detailed explanation-2: -In the well, DNA is a negatively charged molecule due to the phosphate groups that constitute the backbone of DNA. Therefore, by placing the DNA at the negative end of the gel matrix, when the current is turned on the DNA will migrate down the gel towards the positive side because the opposite charges attract.
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