In semi-conservative mode of DNA replication, the short fragments made discontinuously are labelled as Okazaki fragments. These Okazaki fragments need to be matured into continuous DNA strand by which one of the following combination of enzymes?

MOLECULAR BIOLOGY

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Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]

A	DNA Polymerase III and DNA ligase
B	DNA polymerase I and DNA ligase
C	DNA polymerase II and DNA ligase
D	DNA gyrase and DNA ligase

Explanation:

Detailed explanation-1: -What are Okazaki Fragments For? Okazaki fragments are short sections of DNA formed at the time of discontinuous synthesis of the lagging strand during replication of DNA. It is essential as it allows for the synthesis of both the daughter strands required for cell division.

Detailed explanation-2: -Okazaki fragments are short sequences of DNA nucleotides (approximately 150 to 200 base pairs long in eukaryotes) which are synthesized discontinuously and later linked together by the enzyme DNA ligase to create the lagging strand during DNA replication.

Detailed explanation-3: -Lagging-strand replication is discontinuous, with short Okazaki fragments being formed and later linked together.

Detailed explanation-4: -Okazaki fragments are formed on the lagging strand, as DNA polymerase synthesizes a section and then must wait for helicase to open up more of the DNA helix upstream.

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LIFE SCIENCE

OBJECTIVE LIFE SCIENCE

MOLECULAR BIOLOGY