OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Identify the DNA of a person at a crime scene
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Remove a portion of a DNA sample for testing
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Initiate transcription in a cell
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Produce multiple copies of a segment of DNA in laboratory conditions
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Detailed explanation-1: -Polymerase chain reaction, or PCR, is a technique to make many copies of a specific DNA region in vitro (in a test tube rather than an organism). PCR relies on a thermostable DNA polymerase, Taq polymerase, and requires DNA primers designed specifically for the DNA region of interest.
Detailed explanation-2: -The reaction is finally heated to 72oC, at which temperature the Taq enzyme replicates the primed ssDNA (extension). At the end of one cycle, the region between the two primers has been copied once, producing two copies of the original gene region.
Detailed explanation-3: -Polymerase chain reaction (abbreviated PCR) is a laboratory technique for rapidly producing (amplifying) millions to billions of copies of a specific segment of DNA, which can then be studied in greater detail.