OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Template binding
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Catalytic binding
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Promoter binding
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Cation binding
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Detailed explanation-1: -Answer: Explanation: The ’ subunit of polymerase binds to two Zn2+ ions which are thought to participate in the catalytic function of the polymerase. It is also responsible for the binding of the enzyme to the template DNA.
Detailed explanation-2: -The ’ subunit has one insertion, ’ insertion 6 (’i6, residues 942–1129) in the middle of the trigger loop, which plays an important role in all stages of transcription, including the stabilization of the open complex, transcription pausing, and termination (Artsimovitch et al., 2003).
Detailed explanation-3: -The ’ subunit contains structures crucial for transcription, including the sites for ribonucleotide addition and catalysis. Double-stranded DNA enters RNAP through the active site channel, while ribonucleotides (NTPs) enter through the secondary channel.
Detailed explanation-4: -Bacterial RNAP subunit , which is the second-largest subunit and which also is involved in catalysis, corresponds to archaeal RNAP subunit RpoB (or RpoB′/RpoB") and eukaryotic RNAP I, II, and III subunits RPA2, RPB2, and RPC2.