OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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What is the difference in pieces between the following scenarios ____ a circular plasmid cut three times VS a linear (straight line) DNA cut three times
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1
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2
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3
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Explanation:
Detailed explanation-1: -Plasmid DNA is linear in shape whereas chromosomal DNA can be linear or circular in shape. Naturally, plasmid DNA is present as a tightly supercoiled circle to allow itself to fit inside the cell. Chromosomal DNA is often observed as X-shaped structures.
Detailed explanation-2: -A restriction enzyme is a DNA-cutting enzyme that recognizes a specific target sequence and cuts DNA into two pieces at or near that site.
Detailed explanation-3: -On cleaving a circular DNA 3 fragments of DNA will be obtained as there are 3 recognition sites for restriction enzyme.
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