OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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What is the goal of bacterial transformation?
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grow bacteria
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cut DNA into fragments and compare fragment sizes to determine genotype
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amplify a small amount of DNA
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insert a desired gene into a plasmid and insert it into a bacterium
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Explanation:
Detailed explanation-1: -Bacterial transformation is a key step in molecular cloning, the goal of which is to produce multiple copies of a recombinant DNA molecule. Prior steps for creating recombinant plasmids are described in traditional cloning basics and involve insertion of a DNA sequence of interest into a vector backbone.
Detailed explanation-2: -If a plasmid contains the right control sequences, bacteria can be induced to express the gene it contains when a chemical signal is added. Expression of the gene leads to production of mRNA, which is translated into protein.
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