OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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It restricts the number of base pairs that can be tested in a sample.
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It makes the testing simpler by moving the strands into the gel faster.
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It allows the DNA strands to be charged so the electrical current can send the strands into the gel.
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It allows the strands of DNA to be cut into various lengths for testing.
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Detailed explanation-1: -What is the primary purpose of using restriction enzymes prior to gel electrophoresis? It allows the scientist to start with a very small sample of DNA. It allows the strands of DNA to be cut into various lengths for testing. It allows the DNA to be visible on the gel.
Detailed explanation-2: -A restriction enzyme is a protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end. The use of restriction enzymes is critical to certain laboratory methods, including recombinant DNA technology and genetic engineering.
Detailed explanation-3: -Restriction enzymes are molecular scissors that cut DNA on specific sites. All DNA samples are cut with the same restriction enzyme. The obtained fragments are used to get DNA profiling. Gel electrophoresis is the technique by which the DNA can be estimated and analyzed in the form of bands.