OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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D-loop Replication
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Rolling Circle Replication
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Theta model Replication
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None of the above
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Detailed explanation-1: -This is solved, in circular plasmids, by two main strategies: (i) opening of the strands followed by RNA priming (theta and strand displacement replication) or (ii) cleavage of one of the DNA strands to generate a 3′-OH end (rolling-circle replication).
Detailed explanation-2: -This result, which is indicative of semiconservative rolling circle replication of the circular plasmid, is the same as that previously observed with extracts of insect cells multiply infected with baculoviruses recombinant for the HSV-1-en-coded DNA polymerase, UL42 protein, helicase-primase, and ICP8 (8).
Detailed explanation-3: -Well-known replication systems of circular plasmids include theta-type replication, rolling-circle replication, and strand displacement-type replication (del Solar et al., 1998).