OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Saccharomyces
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Rhizobium
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Mycobacterium
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Escherichia
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Detailed explanation-1: -Nowadays, recombinant human insulin is mainly produced either in E. coli or Saccharomyces cerevisiae.
Detailed explanation-2: -Genetically engineered insulin or humulin can be produced by inserting the human gene of insulin into a plasmid isolated from E. coli bacterium and then production of a number of copies or clones of that recombinant gene by insertion of the recombinant gene into another E. coli bacterium.
Detailed explanation-3: -As pictured, 2 E. coli strains were engineered to contain chemically synthesized insulin A or B chain genes inserted into the -galactosidase gene (-gal) of a plasmid cloning vector. The bacteria made a fused, chimeric protein--galactosidase linked by methionine to an insulin tail.