LIFE SCIENCE

Detailed explanation-1: -Enzyme concentration does not affect the enzyme activity directly as substrate concentration determine it until the presence of the substrate in an excess amount.

Detailed explanation-2: -That’s because enzymes don’t affect the free energy of the reactants or products. Instead, enzymes lower the energy of the transition state, an unstable state that products must pass through in order to become reactants.

Detailed explanation-3: -Enzyme activity can be affected by a variety of factors, such as temperature, pH, and concentration.

Detailed explanation-4: -Enzyme activity is affected by a number of factors including the concentration of the enzyme, the concentration of the substrate, the temperature, the pH, and the salt concentration.