OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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plasmid & genes are cut
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cut DNA joined with ligase to make rDNA plasmids
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plasmid & genes are cut
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cut DNA joined with ligase to make rDNA plasmids
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Detailed explanation-1: -Answer: Option 1 is the correct sequence. Plasmid and gene of interest are cut using restriction enzymes and ligated using DNA ligase. This becomes a rDNA.
Detailed explanation-2: -(1) Isolate the DNA sequence gene of interest. (2) Insert your DNA of interest into the vector plasmid: (a) cut both vector DNA and gene of interest with the same restriction enzymes, (b) mix the DNAs: they join by base pairing, (c) add DNA ligase to bond the pieces covalently. The result is a recombinant plasmid.
Detailed explanation-3: -Bacteria can take up foreign DNA in a process called transformation. Transformation is a key step in DNA cloning. It occurs after restriction digest and ligation and transfers newly made plasmids to bacteria. After transformation, bacteria are selected on antibiotic plates.
Detailed explanation-4: -Isolation of genetic material i.e; isolation of the DNA fragments or genes to be cloned. Restriction enzyme digestion. Amplification. Ligation. Insertion of recombinant DNA into host i.e; insertion of the isolated gene in a suitable plasmid vector. More items