OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
You have given bacteria a plasmid with an ampicillin resistance gene. Which of the following results would indicate that transformation of the bacteria occurred?
|
|
Growth in agar without the ampicillin by bacteria with the plasmid
|
|
|
Growth in ampicillin agar by bacteria with the plasmid
|
|
|
Growth in ampicillin agar by bacteria without plasmid
|
|
|
Growth in agar without the ampicillin by bacteria without the plasmid
|
Explanation:
Detailed explanation-1: -Ampicillin is an antibiotic and works by preventing E. coli from constructing cell walls, thereby killing the bacteria. When the ampicillin-resistance gene is present, it directs the production of an enzyme that blocks the action of the ampicillin, and the bacteria are able to survive.
Detailed explanation-2: -The best test would be to take some of the bacteria growing on the LB plate and streak them on an LB/amp plate. If the bacteria are viable on the LB/amp plate, then they are resistant to ampicillin. If no bacterial colonies survive, then they were not ampicillin resistant (they were ampicillin sensitive).
There is 1 question to complete.