MCQ IN MEDICINE

Detailed explanation-1: -T and B lymphocytes are difficult to distinguish morphologically even with electron microscopy, and antibodies are generally used to make the distinction. A specific reagent, consisting of nonionic and cationic detergents, is used for leukocyte differentiation using the Sysmex automated blood analyzer.

Detailed explanation-2: -From the morphological point of view, T and B lymphocytes are indistinguishable since they are both small cells (8–10 microns in diameter) and each possesses a large nucleus with dense hetero-chromatin and a cytoplasmic border that contains few mitochondria, ribosomes, and lyzosomes.

Detailed explanation-3: -B and T cells cannot be distinguished in routinely prepared blood smears; this requires the use of immunocytology to detect cell surface markers that are specific for the different B and T cell types.

Detailed explanation-4: -These two cell types can function independently or cooperatively to defend the body against pathogens. T-lymphocytes can be distinguished from other lymphocytes like B cells and natural killer cells (NK cells) by the presence of a T cell receptor (TCR) on the cell surface.