MEDICAL PHYSIOLOGY

PHYSIOLOGY

GASTROINTESTINAL PHYSIOLOGY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
The media used to count the number of all growing bacterial colonies is____
A
PCA agar
B
endo agar
C
In order to Mac Conkey
D
EMB agar
Explanation: 

Detailed explanation-1: -The total number of living aerobic bacteria can be determined using a plate count agar (PCA) which is a substrate for bacteria to grow on. The medium contains casein which provides nitrogen, carbon, amino acids, vitamins and minerals to aid in the growth of the organism.

Detailed explanation-2: -Counting of bacterial colonies Plates with over 200 colonies were usually counted by dividing the plates into equal sectors (from 1/2 up to 1/8). After counting one sector, the count was multiplied with the total number of sectors to estimate whole plate CFU count.

Detailed explanation-3: -[2] MacConkey agar contains the essential nutrients required for microorganism growth.

Detailed explanation-4: -Plate count agar (PCA) is a bacteriological substrate used for the determination of the total number of live, aerobic bacteria in a sample. It is not a selective medium. The amount of bacteria is expressed as colony-forming units per gram (CFU/g), in solid samples and per ml (CFU/ml) in liquid samples.

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