BIOTECHNOLOGY
APPLICATIONS OF BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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single-stranded DNA “overhangs” that can bind to complementary DNA
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smooth cuts that are sticky due to the “goo” left behind by the enzyme
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The two wires (black and red) on a gel box
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The surface of the LB agar plates so that bacteria can grow
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Detailed explanation-1: -A restriction enzyme is a DNA-cutting enzyme that recognizes specific sites in DNA. Many restriction enzymes make staggered cuts at or near their recognition sites, producing ends with a single-stranded overhang. If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase .
Detailed explanation-2: -Restriction endonucleases (13 out of 18 species used for the test) were certified to cleave single-stranded(ss)DNA. Such enzymes as AvaII, HaeII, DdeI, AluI, Sau3AI, AccII, TthHB8I and HapII were newly reported to cleave ssDNA.
Detailed explanation-3: -Restriction enzymes can create fragments with sticky ends, as is the case with the enzyme BamHI, or blunt ends, as with HaeIII (Table 8.1). Double bars indicate the cleavage site in the DNA strand. DNA ligases are used to join the fragments of DNA generated by restriction enzymes.
Detailed explanation-4: -To join sticky ends DNA ligase enzyme is used which acts by catalyzing the formation of a phosphodiester bond. DNA ligases are called Molecular glue since these enzymes are used during replication for joining phosphodiester backbone, used in RDT for joining sticky ends, and also used in damage and repair cells.