NEET BIOLOGY

Detailed explanation-1: -Gel electrophoresis is used to segregate DNA fragments according to mass and size. DNA is negatively charged and it will travel towards the positive electrode. Hence separation will be on the size of the fragments. The smaller DNA molecules move faster and farthest followed by the larger ones.

Detailed explanation-2: -Scientists most commonly use gel electrophoresis to separate DNA fragments. Because DNA is negatively charged, it moves towards the positive electrode in an electric field. Smaller DNA fragments fit more easily through the gel’s web and travel faster and farther than larger pieces of DNA.

Detailed explanation-3: -Gel electrophoresis and DNA Shorter strands of DNA move more quickly through the gel than longer strands resulting in the fragments being arranged in order of size.

Detailed explanation-4: -Small DNA molecules move more quickly through the gel than larger DNA molecules. The result is a series of ‘bands’, with each band containing DNA molecules of a particular size. The bands furthest from the start of the gel contain the smallest fragments of DNA.

Detailed explanation-5: -Circular DNA. Supercoiled (interwound) DNA molecules have more compact conformations than linear DNAs containing the same number of base pairs, and migrate faster than linear DNA [107-110].