Several procedures involved in the creation of a transgenic organism are listed. 1. A specific gene is identified in DNA. 2. The DNA fragment is placed in a plasmid. 3. The DNA fragment of interest is isolated. 4. The plasmid is transferred to a bacterial cell. 5. A plasmid is cut by a restriction enzyme. Which selection represents the correct order of events in creating a transgenic organism?

BIOTECHNOLOGY

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Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]

A	1, 3, 2, 4, 5
B	3, 5, 2, 4, 1
C	1, 3, 5, 2, 4
D	3, 1, 4, 2, 5

Explanation:

Detailed explanation-1: -The three principal methods used for the creation of transgenic animals are DNA microinjection, embryonic stem cell-mediated gene transfer and retrovirus-mediated gene transfer.

Detailed explanation-2: -Genetic engineering is accomplished in three basic steps. These are (1) The isolation of DNA fragments from a donor organism; (2) The insertion of an isolated donor DNA fragment into a vector genome and (3) The growth of a recombinant vector in an appropriate host.

Detailed explanation-3: -The key steps involved in genetic engineering are identifying a trait of interest, isolating that trait, inserting that trait into a desired organism, and then propagating that organism.

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