GENETICS AND EVOLUTION
MOLECULAR BASIS OF INHERITANCE
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Okazaki Fragments, DNA Ligase
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Leading Strands, DNA Ligase
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Helicase, DNA Ligase
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Histones, DNA Ligase
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Detailed explanation-1: -Okazaki fragments are short sequences of DNA nucleotides (approximately 150 to 200 base pairs long in eukaryotes) which are synthesized discontinuously and later linked together by the enzyme DNA ligase to create the lagging strand during DNA replication.
Detailed explanation-2: -DNA ligases are best known for their role in joining adjacent Okazaki fragments at the lagging strand of the replication fork; however, they are essentially involved in any process that requires sealing of phosphodiester bonds from the DNA backbone.
Detailed explanation-3: -We suggest that DNA ligase I is recruited to the nick between Okazaki fragments by binding to PCNA, which is left in this position after the completion of Okazaki fragment DNA synthesis by the PCNA–DNA pol complex.
Detailed explanation-4: -Okazaki segments are joined by DNA ligase.