GENETICS AND EVOLUTION
MOLECULAR BASIS OF INHERITANCE
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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okazaki fragments
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restriction fragments
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ligase
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SSB proteins
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Detailed explanation-1: -Why can’t the lagging strand be replicated as a continuous segment by DNA polymerase? DNA polymerase can only synthesize in the 5’ to 3’ direction.
Detailed explanation-2: -DNA polymerase proceeds in the 5’ to 3’ direction down DNA. This means the leading strand will be synthesized continuously, as the replication fork opens at the 3’ end. The lagging strand, however, will be synthesized in discontinuous fragments called Okazaki fragments.
Detailed explanation-3: -Answer and Explanation: The production of Okazaki fragments in the lagging strand during replication of DNA is necessary because the new strand synthesis happens in the direction of 3’ to 5’ direction. Still, the DNA polymerase can synthesize the strand only in the 5’ to 3’ direction.
Detailed explanation-4: -The purpose of Okazaki fragments is to allow DNA polymerase to synthesize the lagging strand in segments, since it is not oriented correctly for continuous synthesis.