NEET BIOLOGY

Detailed explanation-1: -There is substantial biochemical evidence indicating that DNA ligase I, which is one of four DNA ligases in mammalian cells (1, 2), is the enzyme responsible for joining Okazaki fragments.

Detailed explanation-2: -On the lagging strand, DNA synthesis restarts many times as the helix unwinds, resulting in many short fragments called “Okazaki fragments.” DNA ligase joins the Okazaki fragments together into a single DNA molecule.

Detailed explanation-3: -There is compelling evidence that DNA ligase I is predominantly responsible for joining Okazaki fragments generated by discontinuous DNA synthesis on the lagging strand at the replication fork.

Detailed explanation-4: -DNA replication on the lagging strand produces numerous fragments called Okazaki fragments that are not joined by their sugar-phosphate backbone. The enzyme DNA ligase joins these fragments to produce a single, continuous strand of DNA.