NEET BIOLOGY

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GENETICS AND EVOLUTION

MOLECULAR BASIS OF INHERITANCE

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?I. Transform bacteria with a recombinant DNA molecule.II. Cut the plasmid DNA using restriction enzymes.III. Extract plasmid DNA from bacterial cells.IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.V. Use ligase to seal plasmid DNA to nonplasmid DNA.
A
III, II, IV, V, I
B
I, II, IV, III, V
C
III, IV, V, I, II
D
II, III, V, IV, I
Explanation: 

Detailed explanation-1: -(i) Transformbacteria with recombinant DNA molecule. (ii) Cut the plasmid DNA using restriction enzymes. (iii) Extract plasmid DNA from bacterial cells. (iv) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.

Detailed explanation-2: -First, DNA of interest is inserted into a plasmid and then the recombinant plasmid is inserted into bacteria. The successfully transformed bacteria are selected and grown to produce the protein of interest. Gene cloning is the process of isolating and making copies of a gene.

Detailed explanation-3: -The correct option is B I → V → II → IV → III. Recombinant DNA technology is the set of techniques which enables the DNA from varied sources to be identified, isolated and recombined so that new characteristics can be introduced into an organism.

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