HISTOLOGY
CONNECTIVE TISSUE
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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What do we use instead of H and E stain to see a Reticular fibers?
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Silver salts
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Formaline
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Iodine salts
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Trichome stain
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Iron-Hematoxylin
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Explanation:
Detailed explanation-1: -Reticulin stains use silver and rely on the argyrophilic properties of the fibers. Argyrophilic cells can adsorb silver but cannot reduce it.
Detailed explanation-2: -Reticular fibers are usually observed as a delicate meshwork of fine fibrils stained black by the silver impregnation method.
Detailed explanation-3: -Deparaffinize and hydrate to distilled water. Oxidize with acidified potassium permanganate for 3 minutes. Rinse with three changes of distilled water. Reduce with 1% potassium metabisulfite for 1 minute. Wash with running tap water for three minutes and rinse with four changes of distilled water. More items
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