BIOMOLECULES AND ENZYMES

Detailed explanation-1: -Denaturation refers to the physical changes that take place in a protein exposed to abnormal conditions in the environment. Heat, acid, high salt concentrations, alcohol, and mechanical agitation can cause proteins to denature.

Detailed explanation-2: -Denaturation defines the unfolding or breaking up of a protein, modifying its standard three-dimensional structure. Proteins may be denatured by chemical action, heat or agitation causing a protein to unfold or its polypeptide chains to become disordered typically leaving the molecules non-functional.

Detailed explanation-3: -Hence, the correct answer is ‘Distilled water’.

Detailed explanation-4: -There are variety of reagents and conditions which can protein denaturation, such as urea, temperature, pH, alcohol, heavy metal, etc. Heavy metal salts present in the vicinity of a protein can induce protein denaturation by disturbing the disulfide bond.