BIOMOLECULES AND ENZYMES

Detailed explanation-1: -Copper sulphate (CuSO4), sodium hydroxide (NaOH), and sodium-potassium tartrate are used to make the biuret reagent (also known as Rochelle salt).

Detailed explanation-2: -The Biuret reagent is a solution composed of sodium hydroxide (NaOH), hydrated copper (II) sulfate, and potassium sodium tartrate.

Detailed explanation-3: -A mixture of 2% copper sulphate solution and 10% sodium hydroxide solution is used to test protein in the food sample. Formation of purple or violet colour confirms the presence of protein in the sample. This test is also called as “Biuret Test".

Detailed explanation-4: -The reagent used in the Biuret Test is a solution of copper sulfate (CuSO4) and sodium hydroxide (NaOH). The NaOH is there to raise the pH of the solution to alkaline levels; the crucial component is the copper II ion (Cu2+) from the CuSO4.

Detailed explanation-5: -The Biuret reagent is made of potassium hydroxide (KOH) and copper (II) sulfate (CuSO4), together with potassium sodium tartrate (KNaC4H4O6·4H2O). The blue reagent turns violet in the presence of proteins, and changes to pink when combined with short-chain polypeptides.