BIOMOLECULES AND ENZYMES

Detailed explanation-1: -The most common -amino-protecting groups for solid-phase peptide synthesis (SPPS) are the 9-fluorenylmethoxycarbonyl (Fmoc) and the tert-butyloxycarbonyl (Boc) groups, used in the Fmoc/tert-butyl (tBu) and Boc/benzyl (Bn) strategies, respectively.

Detailed explanation-2: -The amino group is protected by forming a Boc derivative. These two protected amino acids are joined in an amide bond using dicyclohexylcarbodiimide (DCCI). Continued extension of the peptide is accomplished by hydrolysis of the Boc group using trifluoroacetic acid.

Detailed explanation-3: -The most popular choice of protecting group for amine nitrogen is the carbamate functional group.

Detailed explanation-4: -In order to control the coupling reaction, it is necessary to use protecting groups. By protecting the amine group of one component and the carboxylic acid group of the other, a specific amide bonds can be formed.