BIOMOLECULES AND ENZYMES

Detailed explanation-1: -Solid phase peptide synthesis is traditionally carried out in the C → N direction. The majority of peptides are being synthesized as C-terminal acids or amides. For synthesis of C-terminal modified peptides one can take advantage of many linkers that are available (Guillier et al., 2000).

Detailed explanation-2: -Solid-phase synthesis is the synthesis of chemical compounds whereby the reactant molecule is chemically bound to an insoluble material and reagents are added in the solution-phase. Excess reagents can be easily removed, and purification achieved, by washing.

Detailed explanation-3: -The Group-Assisted Purification (GAP) strategy has been successfully utilized for the solution phase synthesis of N-protected amino acids and peptides by avoiding the use of traditional purification protocols such as chromatography and recrystallization.

Detailed explanation-4: -The two major chemical techniques for peptide production are SPPS and solution phase synthesis (SPS). Classical SPS is based on the coupling of single amino acids in solution. The fragment condensation method has been used for the synthesis of long peptides.

Detailed explanation-5: -10.4 presents a typical scheme of solid-phase synthesis steps [76]. First, C-terminal of the initial amino acid is covalently attached to an insoluble polymeric support.