BIOMOLECULES AND ENZYMES

Detailed explanation-1: -Amino acids are colorless compounds. In order to see the spots on the chromatogram, you will apply a solution of ninhydrin to the paper. Ninhydrin will react with the amino acid to produce a purple compound. Silica gel will serve as the stationary phase in the thin layer chromatography procedures.

Detailed explanation-2: -Methyl Cellosolve (the monomethyl ether of ethylene glycol) has been widely used as the organic solvent in nin-hydrin reagents for amino acid analysis; it has, however, properties that are disadvantageous in a reagent for everyday employment.

Detailed explanation-3: -LIQUID CHROMATOGRAPHY | Biotechnology Applications Ninhydrin has been the standard reagent for detecting amino acids for over 50 years.

Detailed explanation-4: -Thin layer chromatography is an important tool for detecting amino acids by variety of spray reagents. Among these ninhydrin is the most popular due to its high sensitivity. However, ninhydrin produces the same purple/violet color with most amino acids.

Detailed explanation-5: -Ninhydrin reagent, which reacts with amino acids in the oil from fingers to produce a blue-purple stain pattern, was an early addition to fingerprint identification. Other chemical methods have followed, including fuming an object with iodine vapor, treating with silver nitrate, or forming metal complexes.