BIOMOLECULES AND ENZYMES

Detailed explanation-1: -Sanger’s reagent is used for determining the N-terminal amino acid in polypeptide chains, in particular insulin. Sanger’s reagent reacts with amino groups in amino acids to produce dinitrophenyl amino acids. These dinitrophenyl amino acids are stable under acid hydrolysis conditions that break peptide bonds.

Detailed explanation-2: -The reagent l-dimethylaminonaphthalene-5-sulfonyl chloride (dansyl chloride, DNS-C1) reacts with the free amino groups of peptides and proteins as shown in Fig.

Detailed explanation-3: -1-Fluoro-2, 4-dinitrobenzene (commonly called Sanger’s reagent, dinitrofluorobenzene, DNFB or FDNB) is a chemical that reacts with the N-terminal amino acid of polypeptides. This can be helpful for sequencing proteins.

Detailed explanation-4: -1-Fluoro-2, 4-dinitrobenzene 2, Sanger’s reagent (dinitrofluorobenzene or DNFB), used in labeling amines, amino acids, or peptides as the yellow 2, 4-dinitrobenzene (DNB) derivative. Also useful for polypeptide sequencing by identifying the terminal amino acid.