BIOMOLECULES AND ENZYMES

Detailed explanation-1: -The ninhydrin test is a chemical test which is used to check whether a given analyte contains amines or -amino acids. In this test, ninhydrin (a chemical compound with the formula C9H6O4; IUPAC name: 2, 2-dihydroxyindane-1, 3-dione) is added to a test solution of the analyte.

Detailed explanation-2: -The ninhydrin reaction is a qualitative analysis test of hydrocarbons. In this test, we use the reagent known as ninhydrin which is a compound with the formula C9H6O4. This reagent acts as an oxidizing agent for compounds containing an amino group.

Detailed explanation-3: -The ninhydrin test is a chemical test useful to identify ammonia, primary/secondary amines, or amino acids. In this test, ninhydrin reagent is added to the test material, resulting in the production of deep blue colour, also known as Ruhemann’s purple, in the presence of an amino group.

Detailed explanation-4: -The Sakaguchi reagent is used to test for a certain amino acid and proteins. The amino acid that is detected in this test is arginine. Since arginine has a guanidine group in its side chain, it gives a red color with -naphthol in the presence of an oxidizing agent like bromine solution.