BIOMOLECULES AND ENZYMES

BIOLOGY

AMINO ACIDS

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
what is is isoelectric point (pI) of amino acids?
A
pH where there is equal number of zwitter ions and cations
B
pH where there is equal number of anions and zwitter ions
C
pH where the amino acid as zwitter ion carries no net charge
D
pH where the amino acid as zwitter ion carries net charge
Explanation: 

Detailed explanation-1: -The isoelectronic point or isoionic point is the pH at which the amino acid does not migrate in an electric field. This means it is the pH at which the amino acid is neutral, i.e. the zwitterion form is dominant and an amino acids carries no net charge.

Detailed explanation-2: -The isoelectric point (pI) is the pH value at which the molecule carries no electrical charge. The concept is particularly important for zwitterionic molecules such as amino acids, peptides, and proteins. For an amino acid, the isoelectric point is the average of pKa values for the amine and the carboxyl group.

Detailed explanation-3: -Another main property of a Zwitterion is that it has an isoelectric point (represented as pI, pH(I), IEP). This point is the pH value at which the charge in molecules is neutral. Usually, the net charge on a molecule is greatly affected by the pH of its surrounding environment.

Detailed explanation-4: -At pH = 5.02, the pH = pI so the amino acid will exist as the zwitterion with both the positive and negative charges as shown above.

Detailed explanation-5: -The isoelectric point (pI) is the pH at which a particular molecule carries no net electrical charge. The net charge on the molecule is affected by the pH of its surrounding environment and can become more positive or negative due to the gain or loss of protons, respectively.

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