BIOLOGY
AMINO ACIDS
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Sakaguchi test
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Hopkins-Cole test
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Biuret test
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Millon test
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Detailed explanation-1: -The biuret test measures peptide bonds in a sample. Recall that proteins are made up of amino acids connected together with peptide bonds. So the biuret test can’t test for individual amino acids, because those aren’t connected with a peptide bond.
Detailed explanation-2: -The ninhydrin test is a chemical test which is used to check whether a given analyte contains amines or -amino acids. In this test, ninhydrin (a chemical compound with the formula C9H6O4; IUPAC name: 2, 2-dihydroxyindane-1, 3-dione) is added to a test solution of the analyte.
Detailed explanation-3: -The biuret test is a chemical test that can be used to check for the presence of peptide bonds in a given analyte. Therefore, the biuret test can be also be used to gauge the amount of protein present in the analyte.
Detailed explanation-4: -Single amino acids and dipeptides do not give the biuret reaction, but tripeptides and larger polypeptides or proteins will react to produce a light blue to violet complex that absorbs light at 540 nm.