BIOMOLECULES AND ENZYMES

Detailed explanation-1: -Osazone / Kowarsky Test (Phenylhydrazine)

Detailed explanation-2: -Osazone test is a chemical test used to detect reducing sugars. This test even allows the differentiation of different reducing sugars on the basis of the time of appearance of the complex. This test is also termed Phenyl hydrazine test based on the reagent used for this test.

Detailed explanation-3: -Osazone test is performed for each sugar in the boiling water bath and noted down the time for appearing of crystals. Then the shape of osazone of each sugar was examined under microscope. We observed that crystals were appeared at specific interval of time during boiling and cooling slowly after boiling.

Detailed explanation-4: -The Osazone test is a type of biochemical test which is used to detect reducing sugars. This test can distinguish between different types of reducing sugars by the appearance time of the complex. Osazone test can also be called Phenyl hydrozine test depending on the reagent used.