BIOMOLECULES AND ENZYMES

Detailed explanation-1: -The biuret test is a chemical test that can be used to check for the presence of peptide bonds in a given analyte. Therefore, the biuret test can also be used to gauge the amount of protein present in the analyte.

Detailed explanation-2: -Protein electrophoresis is a test that measures specific proteins in the blood. The test separates proteins in the blood based on their electrical charge. The protein electrophoresis test is often used to find abnormal substances called M proteins.

Detailed explanation-3: -Therefore, the biuret test can be also be used to gauge the amount of protein present in the analyte. In this test, the presence of peptides results in the formation of pale purple coloured coordination compounds of the copper (II) ion (when the solution is sufficiently alkaline).

Detailed explanation-4: -The biuret method Copper salts in alkaline solution form a purple complex with substances containing two or more peptide bonds. The absorbance produced is proportional to the number of peptide bonds that are reacting and therefore to the number of protein molecules present in the reaction system.