BIOMOLECULES AND ENZYMES

BIOLOGY

CARBOHYDRATES

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
The following are materials used in the Isolation of Liver Glycogen experiment, EXCEPT:
A
95% Ethanol
B
Sand
C
5% TCA
D
Table sugar
Explanation: 

Detailed explanation-1: -One test for the presence of many simple carbohydrates is to use Benedict’s reagent. It turns from turquoise to yellow or orange when it reacts with reducing sugars.

Detailed explanation-2: -Molisch’s test is a general test for carbohydrates. This test is given by almost all of the carbohydrates. In this test, concentrated sulfuric acid converts the given carbohydrate into furfural or its derivatives, which react with -naphthol to form a purple coloured product. The chemical reaction is given below.

Detailed explanation-3: -Barfoed’s test is a chemical test used for detecting the presence of monosaccharides. It is based on the reduction of copper(II) acetate to copper(I) oxide (Cu2O), which forms a brick-red precipitate. (Disaccharides may also react, but the reaction is much slower.)

Detailed explanation-4: -Starch and glycogen are made up of -glucose subunits, whereas cellulose is made up of -glucose subunits. Cellulose is unbranched and a straight-chain polymer of glucose, whereas starch and glycogen are branched.

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