BIOMOLECULES AND ENZYMES

Detailed explanation-1: -Barfoed’s test is a chemical test used for detecting the presence of monosaccharides. It is based on the reduction of copper(II) acetate to copper(I) oxide (Cu2O), which forms a brick-red precipitate. (Disaccharides may also react, but the reaction is much slower.)

Detailed explanation-2: -Actually when sucrose is heated with Barfoed’s reagent for longer period (> 20 minutes), it may be hydrolyzed (due to acidic medium of the reagent) and the resulting monosaccharides give positive test. This is the reason why non-reducing disaccharide sucrose may also give positive test with Barfoed’s reagent.

Detailed explanation-3: -Here we will explain all the tests so firstly it is Barfoed’s test, it is used to detect only monosaccharide. That is this option is not valid for glucose and fructose.

Detailed explanation-4: -The Barfoed’s test is a test that is used to determine the presence of monosaccharides and disaccharides in a solution and to distinguish between the two. These tests are subject to false positives as sucrose and maltose that are present may result in false positives.